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KMID : 1141020130130020001
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Journal of Applied Oriental Medicine
2013 Volume.13 No. 2 p.1 ~ p.11
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Effects of Mixed Korean Medicinal Water Extract (MAPC) on Lipid Accumulation and Glucose Transport in 3T3-L1 Adipocytes and L6 Skeletal Muscle Cells
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Park Ji-Young
Jeon Seo-Young
Kim Sung-Ok
Lee Eun-Sil
Lee Young-Sun
An Hee-Duk
Kim Mi-Ryeo
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Abstract
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This study was performed to evaluate effect of mixed aqua extract, consist of Mori Folium, Adenophorae Radix, Phllostachyos Folium, Citri Pericarpium (MAPC), on the lipid accumulation in 3T3-L1 adipocytes and glucose transport in L6 skeletal muscle cells. In inducing the differentiation of 3T3-L1 preadipocytes in the presence of an adipogenic cocktail, iso-butylmethylxanthine, dexamathasone, and insulin, we treated with MAPC. MTT assay for measurement of cell viability, oil red o staining for determination of lipid accumulation and western blot for analysis of Glut-4 (glucose transporter 4) protein expression were performed. There was no toxicity on 0, 0.05, 0.1, 0.5, 1 mg/ml of treated MAPC in 3T3-L1 and L6 cells. 0.2 mg/ ml of treated MAPC significantly reduced the lipid accumulation in 3T3-L1 cells compared with that of the same dose of single herbal extracts. And the Glut-4 expression was significantly increased in L6 cells treated with 0.2 mg/ml MAPC compared with that of control. These findings suggest that MAPC may be a potential therapeutic adjunct for obesity and diabetes by targeting the inhibition of adipogenesis in differentiated adipocytes, as well as the translocation of Glut-4 in skeletal muscle cells.
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KEYWORD
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Mori Folium, Adenophorae Radix, Phllostachyos Folium, Citri Pericarpium, lipid accumulation, glucose transport
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